Everything about Brdu totally explained
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Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic
nucleoside which is an
analogue of
thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues.
BrdU can be incorporated into the newly synthesized
DNA of replicating cells (during the
S phase of the cell cycle), substituting for thymidine during
DNA replication.
Antibodies specific for BrdU can then be used to detect the incorporated chemical (see
immunohistochemistry), thus indicating cells that were actively replicating their DNA. Binding of the antibody requires
denaturation of the DNA, usually by exposing the cells to acid or heat.
Because BrdU can replace thymidine during DNA replication, it can cause
mutations, and its use is therefore potentially a health hazard.
Further Information
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